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Evaluation of Nutrient and Anti-Nutrient Characteristics of Different Germplasm of Pongamia Pinnata

M. Pugalenthi, R. N. Deepika, N. Devipriyanka, K. Janardhanan


Two different germplasm of Pongamia pinnata
collected from Omalur, Salem, Tamilnadu and Bharathiar University campus, Coimbatore, Tamilnadu were analyzed for their proximate composition, minerals, seed protein fractionation, amino acid profiles, fatty acid composition of lipids and anti-nutritional substances. Crude protein value was 22.36 g 100 g-1 DM (Omalur germplasm) and 23.69 g 100 g-1 DM (Bharathiar university campus germplasm). The other major nutrient contents (g 100 g-1) were crude lipid 31.06 and 32.16; ash 3.67 and 4.16, total dietary fiber 8.15
and 6.80 and total crude carbohydrate content (NFE) 34.76 and 33.19respectively for Omalur germplasm and Bharathiar university campus germplasm. The seeds were a rich source of minerals such as K, Ca,Mg and P .The amino acids valine, isoleucine, leucine, histidine, lysine were fairly high when compared with the FAO/WHO amino acid recommended pattern. The lipids contained high amounts of unsaturated fatty acids in which linoleic acid was the major fatty acid. The in vitro prote digestibility was found to be 72.34% and 72.16% respectively for Omalur and Bharathiar University Campus germplasm. Antinutritionalsubstances such as total free phenols, tannins, L-DOPA and haemagglutinating activity, were also analyzed.


Proximate Composition, Mineral Composition, Amino Acid Profiles, Fatty Acid Composition, Anti-Nutritional Factors, Germplasm.

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Pugalendhi.M. Deepika.R.N. Devipriyanka.N, Janardhanan.K, (2012.).Evaluation of nutrient and anti-nutrient characteristics of different germplasm of Pongamia pinnata. Proceedings of the International Conference on “Advances in the Electrical and Electronics, Information, Communication and Bioinformatics” (Eds.)

Gunsekaran.N, Beulah Devamalar.P.M. Thulasi Bai.V and Kavya.G. Prathyusha Institute of Technology and Management in association with IEEE, January 24 and 25, 2012. pp. 405-408.

AOAC (1976). Official methods of analysis. Association of official analytical chemists, Washington DC.

Boulter, D. and Derbyshire, E. (1976). The general properties, classification and distribution of plant proteins. In plant proteins, ed. G. Norton. Butterworths, London, UKL, pp 3-24.

Brain, K.R. (1976). Accumulation of L-DOPA in cultures from Mucuna puriens. Plant. Sci. Lett., 7,157-61.

Bray. H.G. and Thorne, W.V. (1954). Analysis of phenolic compounds. Meth. Biochem. Anal., 1, 27-52.

Burns, R.R (1971). Methods for estimation of tannin in grain. Sorghum Agron. J., 63, 511-12.

Dickman, S.R. and Bray, R.H. (1940). Colorimeteric determination of phosphate. Ind. Eng. Chem. Anal. Ed., 12, 665-8.

FAO/WHO (1991). Protein quality evaluation. Food and Agricultural Organization of the United Nations, Rome, Italy. p.66

Folch, J., Lees, M. and Solane-Stanely, G.H. (1957). A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem., 226, 497-506.

Hsu.H.W, Vavak.D.L, Satterlee.L.D and Miller.G.A. (1977).A multienzyme technique for estimating protein digestibility.J.Food Sci, 42, 1269-1271.

Humphires, E.C. (1956). Mineral composition and ash analysis. In Modern Methods of Plant Analysis, Vol. 1, ed. K. Peach and M.V. Tracey. Springer Verlag, Berlin, Germany, pp.468-502.

Issac, R.A. and Johnson, W.C. (1975). Collaborative study of wet and dry technique for the elemental analysis of plant tissue by Atomic Absorption spectrophotometer.J. AOAC, 58,436.

Jambunathan, R. and Singh, U. (1980). Studies on Desi and Kabuli chickpea (Cicer arietinum) cultivars. 1. Chemical composition. In Proc. International. Workshop on chickpea Improvement, 28 Feb-2 March 1979. ICRISAT, Hyderabad, India.

Janardhanan, K., Vadivel, V and Pugalenthi, M. (2003).Biodiversity in Indian under-exploited /tribal pulses-A Review. In: Biotechnology for the Improvement of Legumes,(eds). Jaiwal, P.K and Singh, R.P, Kluwer Academic publishers . Dordretch/ Boston /London.

Khan, A.M., Jacobsen, I. and Eggum, O.B. (1979). Nutritive value of some improved varieties of legumes. J. Sci... Food. Agric., 30, 395-400.

Kuzayli, M.V., Cowan, J.W. and Sabry, Z.I. (1966). Nutritive value of Middle Eastern food stuffs. II. Composition of pulses, seeds, nuts and cereal product of Lebanon. J.Sci. Food. Agric., 17, 82-4.

Liener, I.E. (1976). Phytohaemagglutinins (phytolectins), Ann. Rev. Plant. Physiol., 27, 291-319.

Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951). Protein measurement with folin phenol reagent. J.Biol. Chem. ., 193, 265-75.

Metcalfe, L.D., Schemitz, A.A. and Pelka, J.R. (1966). Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis, Anal. Chem., 38, 514-15.

Muller, H.G. and Tobin, G. (1980). Nutrition and Food Processing. Groom Helm Ltd, London, UK.

Murray, D.R. (1979). The seed proteins of Kowhai, Sophora microphylla AIT. Z. Pflanzen physiol., 93, 423-8.

Nwokolo, E. (1987). Nutritional evaluation of pigeonpea meal. Plant. Foods. Hum. Nutr. 37, 283-90.

Omogbai.F.E, (1990).Lipid composition of tropical seeds used in Nigerian diet.J.Sci.Food Agric 50,253-255.

Pugalenthi.M (2001).Biochemical studies in certain tribal pulses from Western Ghats and Eastern Ghats, South India, Ph.D thesis, Bharathiar University, Coimbatore, India.

Rajaram, N. and Janardhanan, k. (1990). Chemical analysis and nutritional evaluation of certain under exploited Vigna spp. Food Sci. Nutr., 42,213-21.

Rao.P.U and Belavady.B (1979).Chemical composition and biological evaluation of Goa bean (Psophocarpus tetragonolobus) and their tubers .J.Plant Food 3,167-174

Siddhuraju, P., Vijayakumari.K, Janardhanan.K, (1992).Nutritional and chemical evaluation of raw seeds of the tribal pulse Vigna trilobata (L.) Verdc. Inter. J. Food Sci. Nutr. 43,97-103.

Sakla.A.B, Ghali, Y., El-Farra, A. and Rizk.L.F, 1988.The effect of environmental conditions on the chemical composition of soybean seeds: Relationship between the protein, oil, carbohydrate and trypsin inhibitor content. Food chem. 29,221-231.

FAO/WHO/UNU,1985.Energy and protein requirements. Report of a joint FAO/WHO/UNU expert consultation, WHO technical report series, 724.World Health Organization, Geneva, Switzerland.

NRC/NAS.1980.National Research Council Committee on Dietary Allowances.10th edn. National Academy of Science Press, Washington DC. USA.

Liener, I.E.1973.Toxic factors associated with legume proteins. Indian .J. Nutr.Dietet, 10,303-322.

Jaffe, W.G.1980. Phytohaemagglutinins. Toxic constituents of plant food stuffs. 2nd edition. (Eds.). Liener.I.E. Academic Press, New York, USA. pp 69-101.

Maxon.E.D. and Rooney.L.W (1972). Two methods of tannin analysis for Sorghum bicolor (L.). Moench, grain. Crop.Sci.12, 253-254.

Sadasivam and Manickam, A. (1992a). Phenolics .In: Biochemical methods for Agricultural Sciences. Wiley Eastern Ltd, New Delhi, India.pp.187-188.


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