Studies on Anticoagulant Activity of Chitosan Nanoparticle against Some Clinical Pathogens

Abstract

-Gram-negative bacteria interact more strongly with chitosan and therefore have stronger antimicrobial activity. Chitosan nanoparticles were prepared by the ion gel method. The ion gel method involves the formation of chitosan nanoparticles based on electrostatic interactions between the amine groups of chitosan and the negatively charged polyanionic groups. Characterization of nanoparticles is based on size, shape, and surface charge using advanced microscopy techniques such as Fourier Transform Infrared Spectroscopy (FTIR) Scanning Electron Microscopy (SEM) and Ultraviolet Visible Spectroscopy. Chitosan has a wide range of activity and high killing rates against gram-positive and gram-negative bacteria. Chitosan acts as a chelating agent and can inhibit toxin production and microbial growth by selectively binding trace metals. Chitosan's antibacterial mechanism is different from Gram-positive and Gram-negative bacteria. Studies on anticoagulant activity have shown that chitosan sulfate with reduced molecular weight (9-35 kDa) regularly increases anti-Xa activity, which is the same as heparin.