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Protective Role of Sphaeranthus Amaranthoides against Antidiarrhoeal, Antibacterial and Antipyretic Activity in Wister Rats- using of Column Cromotography Extract and Lorke Method

L. Swarna Latha, P. Neelakanta Reddy


Consumption of medicinal herbs is tremendously increasing in order to identify alternative approaches to improve the quality of life and maintain a good health for the past 10 years. Antibiotics provide the main basis for the therapy of bacterial infections. The preliminary phytochemical study, in vitro antibacterial activity and antidiarrhoeal activity of the ethanolic extract of Spheranthus amaranthoides having ethnomedicinal uses were investigated. The preliminary phytochemical analysis of the extracts revealed the presence of flavonoids, carbohydrate, tannins, saponins, steroids, glycosides, terpenoids and alkaloids. The extracts were subjected for screening of in vitro antibacterial activity against selected major disease causing pathogens viz. Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilis, Enterococcus faecalis and Escherichia coli at the concentrations of 50, 100, 200, and 400μg/ml by disc diffusion assay method. The results of antibacterial activity revealed good inhibitory activity against all the tested pathogens. Spheranthus amaranthoides also caused a significant (p<0.001) castor oil induced antidiarrhoeal effect in rats. The results suggest that these activites might be due to the presence of the potent phytochemicals in the extract of Spheranthtus amaranthoides. But despite effort to maintain good health, man and animals alike still confront disease conditions which are due to exposure to physiopathological agent and medicinal plants such as Sphaeranthus amaranthoides can be used as a therapeutic agent.
The present investigation clearly reveals the antibacterial nature of this plant and suggests that this plant could be exploited in the management of diseases caused by these bacteria in human. Further ethanolic extract of SPA in graded doses reduced diarrhoea and thereby lending support to use SPN in folklore medicine against chronic diarrhea. The phytochemical analysis revealed that the active principle, which is a phenolic compound present in the ethanolic extract of SPA is thus responsible for the antibacterial and antidiarroheal activity. So it has become expedient to examine scientifically the protective effects of these herbal plants and thus there has been a continuing search for new and more potent antibiotics (Heisig, 2001). Therefore there is an increased need for research in the investigations on plants as a source of human disease management (Aiyelagabe et al. 2000; Mouniswamy et al. 2002; Woldemichael et al. 2003In addition, The ethanol extract of sphaeranthus amaranthoides was investigated for antipyretic activity in rats using yeast. In rats, yeast (20ml/kg body weight, s.c) caused elevation of rectal temperature of 0.50°c 18h after administration.The extract (100and 200 mg/kg body weight, s.c) produced a significant (p<0.05) dose dependent inhibition of temperature elevation. ). It is also observed that the role of mathematical models in biological and clinical research is vital in nature to ensure the accuracy and the effectiveness of the drug developed.


Sphaeranthus Amaranthoides, Antidiarrhoeal, Antibacterial, Yeast, Antipyretic.

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Mandal,S.C, Mukherjee, P.K., Seha, K., Pal.M and Saha, B.P (1997). Antidiarrhoeal evaluation of Ficus racemosa Linn. Leaf e extract. Nat.Prod.Sci. 3:100-103.

Morebise O, Fafunso MA, Makinde JM, Olajide OA and Awe EO (2002) Antiinflammatory property of the leaves of Gongronema latifolium. Phytother Res. 16, S75-77 Aiyelagabe, 0-0(2000). Antibacterial activity of Jatropha multifida roots. Fitoterapia 72:544-546

Arokiyaraj S, Radha R, Martin S and Perinbam K (2008) Phytochemical analysis and anti-diabetic activity of Cadaba fruticosa R.Br. Indian J. Sci.Technol. 1 (6), 1-4. Domain site:

Bisignano G, Sanogo R, Marino A, Aquino R, D'Angelo V, Germanò MP, De Pasquale R and Pizza C (2000) Antimicrobial activity of Mitracarpus scaber extract and isolated constituents. Lett. Appl. Microbiol. 30, 105-108.

Chellaram C and Edward JKP (2009) Anti-inflammatory potential of coral reef associated gastropod, Drupa margariticola. Indian J. Sci.Technol. 2 (2), 75-77. Domain site:

Collins CH, Lyne PM. In: Microbiological Methods. 7th ed. London: Butterworths; 1995.

Court WE, Evans WC and Trease GE (1957) The structure of the root and stem of Rauwolfia caffra Sond. J. Pharm. Pharmacol. 9, 237-250.

Devi BP, Boominathan R and Mandal SC (2002) Evaluation of anti-diarrheal activity of Cleome viscosa L. extract in rats. Phytomedicine. 9, 739-742.

Ferreira SH, Herman A and Vane JR (1972) Proceedings: Prostaglandin generation maintains the smooth muscle tone of the rabbit isolated jejunum. Br. J. Pharmacol. 44, 328P-329P.

Galvez J, Zarzuelo A, Crespo ME, Lorente MD, Ocete MA and Jiménez J (1993) Antidiarrhoeic activity of Euphorbia hirta extract and isolation of an active flavonoid constituent. Planta Med. 59, 333-336.

Gangadevi V, Yogeswari S, Kamalraj S, Rani G and Muthumary J (2008) The antibacterial activity of Acalypha indica L. Indian J. Sci.Technol. 1 (6), 1-5. Domain site:

Gordon DM (2001) Geographical structure and host specificity in bacteria and the implications for tracing the source of coliform contamination. Microbiology. 147, 1079-1085.

Hammer KA, Carson CF and Riley TV (1999) Antimicrobial activity of essential oils and other plant extracts J. Appl. Microbiol. 86, 985-990.

Heisig P (2001) Inhibitors of bacterial topoisomerases: mechanisms of action and resistance and clinical aspects. Planta Med. 67, 3-12

Chattopadhyay, D., G. Arunachalam, L. Ghosh, K. Rajendran, A.B. Mandal and S.K.Bhattacharya, 2005. Antipyretic activity of Alstonia macrophylla Wall ex A. DC: An ethnomedicine of Andaman Islands. Journal of Pharmacy and Pharmaceutical Science, 8: 558-564.

Spacer, C.B. and C.D. Breder, 1994. The neurologic basis of fever. New England Journal of Medicine, 330: 1880-1886.

Veugelers, P.J., J.M. Kaldor, S.A. Strathdee, K.A. Page-Shafer, M.T. Schechter, R.A. Coutinho, I.P. Keet and G.J. van Griensven, 1997. Incidence and prognostic significance of symptomatic primary human immunodeficiency virus type 1 infection in homosexual men. Journal of Infectious Disease, 176: 112-117.

Cheng, L., H. Ming-liang and B. Lars, 2005. Is COX-2 a perpetrator or a protector? Selective COX-2 inhibitors remain controversial. Acta Pharmacological Sinica, 26: 926-933.

Kirtikar, K.R., Basu, B.D., 1918. Indian Medicinal Plants. Published by Lalit Mohan Basu, Allahabad, India, pp. 1–2.

Nadkarni, K.M., 1976. Indian Materia Medica, 1. Published by Popular Prekashan, Bombay, p. 1162.

Swarna latha, P.Neelakanta reddy 2009 Antimicrobial Antidiahorreal And Phytochemical Activities Of Sphaeranthus Amaranthoides Indian Jornal Of science and technology, vol.2No.3 (Mar.2009), pp.45-48.

Swarna latha, p. Neelakanta reddy 2009. protective role of sphaeranthus amaranthoides extract on dermal wounds in wistar rats, International journal on Applied Bioengineering, Vol.3, No.1, January 2009. pp.52-55

Lorke D (1983). A new approach to practical acute toxicity testing. Arch. Toxicol. 54: 275-287

Brasseur, T., 1989. Antiinflammatory properties of flavonoids. Journal de pharmacie de Belgique, 44 : 235-241.

Vimala, R., S. Nagarajan, M. Alam, T. Susan and S. Joy, 1997. Anti-inflammatory and antipyretic activity of Michelia champaca Linn. (White variety), Ixora brachiata Roxb. And Rhynchosia cana (wild.) D. C. flower extract.Indian Journal of experimental Biology, 35: 1310- 1314.


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